Product Description
Hygromycin B
CAS:31282-04-9
:C20H37N3O13
:527.52
Hygromycin B, an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.
Application:
Hygromycin B has certain deworming activity, it has good nematode flooding effect if it is added in feed for pigs and poultry for long term, As a repellent resistant drug it can be used in chicken and pig feed.
Assay:90%HPLC
valence:1109u/mg
melting point:160-180ºC
preserving temperature:2~8ºC,light should be avoided
work concentration:
prokaryote(e.g., E. coli, at 100 mg/ml); lower eukaryotes (e.g., yeast, at 200 mg/ml) and higher eukaryotes (e.g., mammalian cells in culture, 150-400 mg/ml)
Hygromycin B is soluble in water, at concentrations >50 mg/mL, and stable in aqueous solution.Solutions should be sterilized by filtration, not autoclaving.
Thermal stability:
Aqueous solutions can remain stable for at least a month from 37ºC to 60ºC.
Mammalian cells: 50-1000ug/mL
Plant cells: 20-200ug/mL
Bacteria: 20-200ug/mL
Fungi: 200-1000ug/mL
Your appropriate concentration should be tested experimentally!
Packing:25kg/fiber paper board drum
China (Mainland)